Polydomous ant species have a nest structure composed of multiple unconnected satellite nests in addition to the main nest containing the queen. These satellite nests often contain workers, brood, and food for the colony. A key piece of information that must be shared among the colony to avoid the breakdown of colony function is the presence of a fertile queen. In many species, workers that are artificially isolate from the queen will activate their ovaries and begin producing male-destined eggs. However, this rarely occurs in wild, queenright colonies. I study the mechanisms used to communicate the presence of a fertile queen to workers in distant nests where workers are isolated from the queen for extended periods of time. Using Novomessor cockerelli, Oecophylla smaragdina, and Camponotus floridanus, I study the behavioral and chemical mechanisms that signal the presence of the queen to her workers. These mechanisms include queen fertility signals, policing, and larval regulation.